| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | Nick Blakey | Unattached | NT | 51.83 | |
| 3 | Jackson Lincoln | SO | Cornerstone |
51.94 | Concordia Legacy Invitational |
| 4 | Miles Gray | FR | Indiana Tech |
49.27 | WHAC Outdoor Track & Field Championships |
| 5 | Naythan Haslam | SR | Siena Heights |
48.99 | SVSU Classic |
| 6 | Adam Bailey | SO | Aquinas |
50.03 | SVSU "Jet's Pizza" Invitational |
| 7 | Satchel Horton | SR | Aquinas |
50.16 | NCC Dr. Keeler Track & Field Meet |
| 8 | Jacob Sand | SO | Siena Heights |
52.01 | 54th Annual Hillsdale College "GINA" Relays |