| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 4 | BASHAM, Emily | Rochester Community | 56.09 |
| 5 | NELSON, Macie | Rochester Community | 55.14 |
| 6 | HACKBUSH , Vada | Ft. Wayne North Side | NT |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 2 | OSBORN, Arianna | Homestead | 53.54 |
| 3 | PARRISH, Ryann | Homestead | 51.30 |
| 4 | SHAW, Alex | Carroll (Fort Wayne) | 50.00 |
| 5 | HANDSHOE, Leah | Leo | 47.10 |
| 6 | SMITH, Adison | Carroll (Fort Wayne) | 48.00 |
| 7 | COOLMAN, Gloria | Leo | 50.50 |
| 8 | HOLZINGER, Emma | Huntington North | 51.44 |
| 9 | BETTERLY, Arianna | Huntington North | 55.10 |