| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Marian (Ind.) |
A | Crayton II | Hamer | Maxey | McDade | 42.73 |
| 2 | Huntington |
A | Meek | Atkins | LaRue | Worsham | 42.50 |
| 3 | Madonna |
A | Cannon Jr. | Cunningham | Hall | Anderson | 42.04 |
| 4 | Indiana Tech |
A | Bloir | Lincoln | Warner | Elkins | 40.40 |
| 5 | Bethel (Ind.) |
A | Ameen | Jackson | Corbitt | Jones | 41.75 |
| 6 | St. Ambrose |
A | Olenek | Reemtsma | Reynolds | Reemtsma | 42.50 |
| 7 | St. Francis (Ill.) |
A | Biosah | Cullison | Giddie | Bailey | 42.68 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 3 | Rochester (MI) |
A | Jenkins | Malinowski | Daniels | Williams Jr | DNS |
| 4 | St. Francis (Ind.) |
A | Malone | Forker | Gordon | Plattner | DQ |
| 5 | Cornerstone |
A | Courtney | Brightmon | Manongo | Dwumfour-Poku | 44.15 |