| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | MARTIN, Kendall | Unattached | 5:00.00 |
| 2 | NAAS, Cara | Unattached | 5:09.00 |
| 3 | GALLAGHER, Bridget | Guerin Catholic | 5:10.00 |
| 4 | KENNEDY, Sophia | Unattached | 5:10.00 |
| 5 | KLOPSTAD, Jasmine | Unattached | 5:19.00 |
| 6 | PARKER, Abby | Unattached | 5:20.00 |
| 7 | TIPMORE, Sophia | North Central (Indianapolis) | 5:30.00 |
| 8 | HEGEDUS, Jessica | Unattached | 5:50.00 |
| 9 | GELLER, Grace | Oak Hill | 6:10.00 |