| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Ind Tech Alumni | A | NT | |||||
| 2 | St. Francis (Ill.) |
A | Biosah | Brown | Cullison | Bailey | 41.53 | ONU Outdoor Open |
| 3 | Marian (Ind.) |
A | Hamer | Thomas | Novotny | Crayton II | 41.24 | Oliver Nikoloff Invitational |
| 4 | Indiana Tech |
A | Warner | Elkins | Lincoln | Highsmith | 40.08 | NAIA Outdoor Track & Field Championships |
| 5 | Northwest Kansas | A | Brownridge III | Akpoli | Dotson | Loredo | 40.90 | |
| 6 | Bethel (Ind.) |
A | Ameen | Jackson | Corbitt | Jones | DNS | SWU-Susan Rouse Invite |
| 7 | Indiana Tech Developmental | A | DNS |