| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Grace |
B | Margaritidis | Rufenacht | Helton | Yoder | 3:32.00 | |
| 4 | Huntington |
A | Atkins | Gerke | Meek | Chesterman | DNS | |
| 5 | Bethel (Ind.) |
A | Mott | Stalker | Simpson | Sperry | DNS | Strive for Greatness College |
| 6 | Grace |
A | Smith | Sallee | Beatty | Smith | 3:20.36 | Indiana Little State Championships |
| 7 | Mount Vernon Nazarene |
A | Near | Couchman | Slemmons | Gaines | 3:24.87 | Reggie Thomas Classic- Tiffin |
| 8 | Indiana Kokomo |
A | Phillips | Vogel | Felger | Turner | 3:49.66 | River States Conference Outdoor Track & Field Championships |