| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | WEATHERS, Joye | The Village of Louisville | NT | |
| 2 | WILLIAMS, Autumn | Aln Sports Organization Inc | 33.00 | |
| 3 | HOGAN, Alana | Aln Sports Organization Inc | 30.05 | |
| 4 | GOSS, Breonna | Aln Sports Organization Inc | 28.50 | |
| 5 | CROOKE, Kya | Soca Speed Track Club | 29.00 | |
| 6 | TINCH, Kennedy | Soca Speed Track Club | 31.68 | |
| 7 | ESCOTO, Isabella | Soca Speed Track Club | 34.00 |