| * | IHSAA Boys Automatic Standard | 39.01 |
| Lane | Athlete | Yr. | Team | Seed |
|---|---|---|---|---|
| 2 | Rocky Slone | SO | West Noble | 42.14 |
| 3 | Eduardo Montoya | JR | Lakeland | 42.04 |
| 4 | Jackson Simons | JR | Southwood | 41.38 |
| 5 | Garrett Sauter | SO | Angola | 41.44 |
| 6 | Eli Lootens | SR | Marion | 42.01 |
| 7 | Max Moore | SO | Woodlan | 42.11 |
| 8 | Keith Suttle | JR | FW Northrop | 42.25 |
| Lane | Athlete | Yr. | Team | Seed |
|---|---|---|---|---|
| 1 | Braden Melchi | SR | Homestead | 40.61 |
| 2 | Sammy Sommers | SR | Oak Hill | 40.56 |
| 3 | Ronald Elliot | JR | FW Wayne | 40.19 |
| 4 | Chris Flick | SR | Carroll (Fort Wayne) | 39.48 |
| 6 | Kairal Johnson | SR | Homestead | 39.87 |
| 7 | Nate Burns | JR | Huntington North | 40.32 |
| 8 | Sam Wood | JR | Churubusco | 40.59 |
| 9 | Kaleb Krontz | SO | Garrett | 41.04 |