| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Note |
|---|---|---|---|---|---|---|---|
| 1 | Lebanon | A | Hasty, Katie | Kincaid, Lizzie | Coyle, Abbi | Adams, Murphy | |
| 2 | Western | A | Nicholson, Natalie | Manuel, Sarah | Parr, Madison | Marley, Emma | |
| 3 | Western Boone | A | Mahin, Jenna | Randolph, Jordan | Reid, Naomi | Fuesting, Kate | |
| 4 | Hamilton Heights | A | Roth, Abigail | Christiansen, Abby | Guthrie, Morgan | Mitchell, Maria | |
| 5 | Northwestern | A | Jocius, Sasha | Deck, Ella | Fernandes, Kaylynne | Yager-Motel, Sophia | 10:54.95 Maverick HSR Qualifier |
| 6 | Cass | A | Leicht, Makenna | Forsythe, Grace | Wiles, Jackie | Tigler, Emily | |
| 7 | Maconaquah | A | Alvarado, Isabelle | Klepinger, Emma | Merritt, Alexandra | Salinas, Haley |