| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 2 | Northern Kentucky | A | DNS | Horizon League Outdoor Track & Field Championships |
| 3 | Grand Valley St. | B | 42.25 | GLIAC Outdoor Track & Field Championships |
| 4 | Indianapolis | A | 42.00 | Oliver Nikoloff Invitational |
| 5 | Grand Valley St. | A | DNS | GLIAC Outdoor Track & Field Championships |
| 6 | Miami (Ohio) | B | 41.10 | Oliver Nikoloff Invitational |
| 7 | Miami (Ohio) | A | 41.10 | Oliver Nikoloff Invitational |
| 8 | Indiana East | A | 42.06 | Emory Classic |
| 9 | Indiana Wesleyan | A | 44.06 | Lindsey Wilson Classic |