| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | DE SANTIAGO, Lisbeth | Bloomington North | 1:00.18 | |
| 2 | NJAU, Avery | Bloomington North | 1:00.13 | |
| 3 | TIERNEY, Meghan | Bloomington South | 1:00.08 | |
| 4 | DAVIS, Amiyah | Bloomington South | 1:00.03 | |
| 5 | ROBERTS, Javenique | Bloomington North | 1:00.03 | |
| 6 | MORRISON, Marikate | Bloomington North | 1:00.08 | |
| 7 | STEBBINS, Torie | Bloomington South | 1:00.13 | |
| 8 | AWORI, Katrina | Bloomington North | 1:00.23 |