| # | State Standard | 3:57.93 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Note |
|---|---|---|---|---|---|---|---|
| 1 | Speedway | A | Rendes, Sydney | Moore, Kylie | Sears, Mary | Wachter, Emily | |
| 2 | Covenant Christian (Indpls) | A | Tolley, Mara | Croner, Rachel | Notter, Sophia | Crane, Hannah | |
| 3 | Indianapolis Cardinal Ritter | A | Troll, Christina | Acosta, Isis | Wells, Hannah | Wehrle, Mariah | |
| 4 | Pike | A | Wykle, Makyia | Stanhope, Elizabeth | Ross, Jailyn | Miller, Ja'Niyah | |
| 5 | Perry Meridian | A | Dale, Alexandria | Baird, Caitlin | Bargue, Hannah | Hunter, Iyanla | |
| 6 | Ben Davis | A | Goss, Khera | Coleman, Jada | Cooks, Areiahna | Cole, Monyae | |
| 7 | Southport | A | Stevance, Alicia | Piatt, Kierstin | Manning, Nicole | Edington, Savannah | |
| 8 | Decatur Central | A | Williams, Lorena | Brock, Kelli | Marble, Oleysa | Marble, Vierigien |