| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Note |
|---|---|---|---|---|---|---|---|
| 1 | North Putnam | B | Earley, Megan | Lotzow, Lauren | Neudeck, Hannah | Hill, Tamara | |
| 2 | Warren Central | B | Clarke-bennett, Ayanna | Garrison, Kimberly | Waymire, Brinna | Westerfield, Shelby | |
| 3 | Franklin Central | B | Gammon, Katie | Miller, Elise | Schmeler, Shelley | Simon, Katie | |
| 4 | Avon | B | Brandlein, Jacki | Myers, Elizabeth | Myers, Lauren | Wang, Zoe | |
| 5 | Indianapolis George Washington | B | Daniels, Nancy | Dunkerson, Theresa | Heller, April | Heller, Erin | |
| 6 | Brownsburg | B | Freeman, Kierra | Jennings, Kaylee | Steuerwald, Nina | White, Maddy | |
| 7 | Southport | B | Beikman, Cateline | Karushis, Elizabeth | King, Desyrae | Lindbeck, Caitlin | |
| 8 | Lawrence North | B | McCreary, Kendall | Ouse, Michaela | Ware, Kanasha | Wickham, Summer |